Comparative secondary metabolite expression in callus cultures and mother plant in Barleria prionitis L.

The present study is the first report on quantitative analysis of secondary metabolites in callus cultures Barleria prionitis L. and comparison with mother plant. Callus was obtained from stem internode (on MS medium 0.5 mg l−1 NAA, BAP 300 ascorbic acid), leaf 1.0 2,4-D, BAP, acid ) explants and, multiplied by subculturing thrice at an interval 25 days. Calli plant counterparts were extracted four solvents (methanol, ethanol, acetone, distilled water) examined for active compounds antioxidant potential. not only preserved plant’s metabolite profile but also displayed elevated levels. Leaf-derived surpassed stem-derived most studied parameters. highest total phenolic content (21.46 GAE g−1 FW) flavonoid (24.58 RE observed methanol extract leaf-derived callus, representing a 3-fold 2-fold increase over leaf, respectively. Antioxidant capacity based FRAP DPPH assay (7-fold plant, respectively) while ABTS activity (122-fold increase) acetone callus. HPTLC revealed enhanced concentrations squalene (10-fold) SME (2.3-fold) respectively, compared to explants. results RP-HPLC phenolics showed gallic (99-fold ethanol whereas catechol maximum (37-fold aqueous These findings suggest that B. can be potential source metabolites. Further, cell suspension produced which could avenue large-scale production bioactive compounds. For time, components determined quantitatively